Recipe

Primary Growth Medium

Reagent Final concentration
DMEM/F12     1×
Insulin–transferrin–selenium (ITS)     1×
Epidermal growth factor (EGF) 50 ng/mL
Fetal bovine serum (FBS)    5%
Gentamicin 50 µg/mL
Penicillin/streptomycin     1×
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  1. doi:10.1101/pdb.rec078873 Cold Spring Harb Protoc 2015: pdb.rec078873- © 2015 Cold Spring Harbor Laboratory Press
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