MOPS Buffer (10×)
| Reagent | Quantity (for 1 L) | Final concentration |
|---|---|---|
| MOPS free acid, ultrapure (e.g., Calbiochem ULTROL) | 41.86 g (or 46.26 g of the sodium salt) | 0.2 m |
| Sodium acetate, anhydrous | 4.1 g (or 6.8 g of acetate · 3H2O) | 0.05 m |
| Na2EDTA (0.5 m, pH 7.5) | 20 mL | 0.01 m |
| Bring the pH to 7.0 with NaOH (or if the MOPS sodium salt is used, then use glacial acetic acid to adjust the pH). Do not autoclave. Store in the dark, covered with foil, at room temperature. The solution will turn yellow with time, but this is normal. | ||
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