Cell Lysis Buffer for ChIP-Seq

doi:10.1101/pdb.rec085498

Recipe

Cell Lysis Buffer for ChIP-Seq

50 mm Tris (pH 8.0)

140 mm NaCl

1 mm EDTA

10% glycerol

0.5% NP-40

0.25% Triton X-100

Filter-sterilize. Store for up to 1 yr at 4°C. Add one protease inhibitor tablet or 2 mL of 25× inhibitor stock solution as directed by the manufacturer (cOmplete EDTA-Free; Roche) per 50 mL of buffer immediately before use. In addition, if immunoprecipitating with an antibody against the histone modification H3K27ac, include the histone deacetylase inhibitor sodium butyrate at a final concentration of 5 mm. Other inhibitors are generally not necessary when immunoprecipitating transcription factors but should be considered when using antibodies against posttranslational covalent modifications.