Chloroplast Homogenization Buffer

100 mm Tris-HCl (pH 7.5)

5 mm MgOAc

50 mm KCl

5 mm β-mercaptoethanol

0.7 m sucrose (certified RNase-free)

Prepare in ultrapure (MilliQ) or certified RNase-free water. Filter-sterilize through a 0.22-µm filter.

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