Recipe

French Press Buffer for Prokaryotic Polysomes

20 mm Tris-HCl (pH 7.6)

50 mm MgCl2

150 mm NH4Cl

Prepare in ultrapure (MilliQ) or certified RNase-free water. Filter-sterilize through a 0.22-µm filter.

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  1. doi:10.1101/pdb.rec081554 Cold Spring Harb Protoc 2015: pdb.rec081554- © 2015 Cold Spring Harbor Laboratory Press
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