French Press Buffer for Prokaryotic 70S Ribosomes
20 mm Tris-HCl (pH 7.5)
50 mm MgOAc
100 mm NH4Cl
1.0 mm DTT
0.5 mm EDTA
Prepare in ultrapure (MilliQ) or certified RNase-free water. Filter-sterilize through a 0.22-µm filter.
- © 2015 Cold Spring Harbor Laboratory Press
