High-Salt Sucrose Cushion Buffer for Chloroplast Ribosomes

10 mm Tris-HCl (pH 7.5)

10 mm MgOAc

150 mm KCl

6 mm β-mercaptoethanol

1 m sucrose (certified RNase-free)

Prepare in ultrapure (MilliQ) or certified RNase-free water. Filter-sterilize through a 0.22-µm filter.

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