Plant Sucrose Cushion Buffer
20 mm Tris-HCl (pH 7.6)
5 mm MgCl2
50–200 mm NH4Cl
60% (w/w) sucrose (certified RNase-free)
Prepare in ultrapure (MilliQ) or certified RNase-free water. Filter-sterilize through a 0.22-µm filter.
- © 2015 Cold Spring Harbor Laboratory Press
