Prokaryotic Bind/Wash Buffer
20 mm Tris-HCl (pH 7.5)
100 mm NH4Cl
10.5 mm MgOAc
0.5 mm EDTA
3 mm β-mercaptoethanol
Prepare in ultrapure (MilliQ) or certified RNase-free water. Filter-sterilize through a 0.22-μm filter.
- © 2015 Cold Spring Harbor Laboratory Press
