Recipe

Tyrode's External Solution

Reagent Final concentration (mm)
NaCl  137
KCl   5.4
MgCl2   0.5
CaCl2   1.8
Na-HEPES  11.8
Glucose   10
Adjust the pH of this solution to 7.4 with NaOH. This solution can be prepared the day before the experiment, without glucose, and kept at 4°C until the time of the experiment.

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  1. doi:10.1101/pdb.rec079632 Cold Spring Harb Protoc 2015: pdb.rec079632- © 2015 Cold Spring Harbor Laboratory Press
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