Recipe

G Solution

Reagent Amount Final concentration
HBSS without calcium, magnesium, or phenol red (Life Technologies 14175-079)   1 L
Penicillin-streptomycin (100×; Life Technologies 15140-122)  10 mL   1×
Fungizone (Life Technologies 15290-018)  10 mL   1%
d-Glucose (Sigma-Aldrich G5400)   0.9 g  0.9 g/L
CaCl2 (119 mm) 0.4 mL 47.6 µm

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  1. doi:10.1101/pdb.rec079228 Cold Spring Harb Protoc 2015: pdb.rec079228- © 2015 Cold Spring Harbor Laboratory Press
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