| DMEM/F12 (Life Technologies 11330-032) |
500 mL |
|
| Penicillin-streptomycin (100×; Life Technologies 15140-122) |
5 mL |
1× |
| Nu-serum IV (BD Biosciences 355104) |
25 mL |
5% |
| Bovine pituitary extract (3 mg/mL; BD Biosciences 354123) |
4.2 mL |
25 µg/mL |
| ITS+ Premix (BD Biosciences 354352) |
2.5 mL |
|
| Epidermal growth factor (100 µg/mL; BD Biosciences 354001) |
100 µL |
20 ng/mL |
| Cholera toxin (1 mg/mL; Sigma-Aldrich C8052) |
50 µL |
100 ng/mL |
| 3,3′,5-Triiodo-l-thyronine (50 µm; Sigma-Aldrich T2877)
|
50 µL |
5 nm |
| Dexamethasone (100 mm; Sigma-Aldrich D1756)
|
5 µL |
1 µm |
| d-Glucose (Sigma-Aldrich G5400)
|
2.5 g |
4.7 mg/mL |
| Nicotinamide (Sigma-Aldrich N3376) |
0.66 g |
1.22 mg/mL |
| Soybean trypsin inhibitor (type I; Sigma-Aldrich T6522) |
50 mg |
0.1 mg/mL |
