Lysis Buffer with Protease Inhibitors
| Reagent | Final concentration |
|---|---|
| 2-(N-morpholino)ethanesulfonic acid | 5 mm |
| EDTA | 0.5 mm |
| KCl | 1 mm |
| Sorbitol | 0.6 m |
| Protease inhibitors | |
| Antipain | 1 µg/mL |
| Aprotinin | 1 µg/mL |
| Benzamidine hydrochloride | 0.2 mg/mL |
| Bestatin | 1 µg/mL |
| Chymostatin | 1 µg/mL |
| Leupeptin | 1 µg/mL |
| NaF | 0.2 mg/mL |
| Pepstatin | 1 µg/mL |
| PMSF | 10 µg/mL |
| Prepare the lysis buffer by combining the first four ingredients, add the protease inhibitors, and then adjust the pH to 6 at room temperature with KOH. | |
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