Recipe

SD Medium

17% (w/v) yeast nitrogen base

0.5% ammonium sulfate

0.1% yeast extract

0.3% dextrose

2 mg/mL each of adenine, histidine, tryptophan, and uracil

3 mg/mL each of lysine and leucine

Adjust to pH 6 at room temperature with KOH.

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  1. doi:10.1101/pdb.rec084483 Cold Spring Harb Protoc 2015: pdb.rec084483- © 2015 Cold Spring Harbor Laboratory Press

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