Lysis Buffer for Yeast
| Reagent | Quantity | Final concentration |
|---|---|---|
| Triton X-100 | 10 mL | 2% (v/v) |
| SDS (10%) | 50 mL | 1% (w/v) |
| NaCl (5 m) | 10 mL | 100 mm |
| Tris-Cl (1 m, pH 8.0) | 5 mL | 10 mm |
| EDTA (0.5 m, pH 8.0) | 1 mL | 1 mm |
| Prepare in 500 mL of deionized water. Autoclave for 20 min at 15 psi (1.05 kg/cm2). Store at room temperature; it will keep for at least 1 yr. | ||
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