Synthetic Amino-Acid-Dropout Medium (SD-All)
| Reagent | Quantity | Final concentration |
|---|---|---|
| Difco yeast nitrogen base without amino acids | 6.7 g | 6.7 g/L |
| Agar | 20 g | 20 g/L |
| Dextrose (40%) | 50 mL | 20 g/L |
| Add 950 mL of deionized water to 6.7 g Difco yeast nitrogen base without amino acids and 20 g of agar. Autoclave for 20 min at 15 psi (1.05 kg/cm2). After autoclaving, add 50 mL of a 40% dextrose solution. Cool the medium to ∼60°C and pour into Petri dishes (∼25 mL per 100-mm plate). Store the SD-all plates at 4°C; they will keep for at least 6 mo. | ||
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