Recipe

Synthetic Complete (SC) Medium, Minus Adenine, Plus Methotrexate

Reagents Final concentration (1×)
Yeast nitrogen base (YNB) medium without amino acids or ammonium sulfate (Bioshop)  1.74 g/L
Noble agar (purified agar; Bioshop)   4% (w/v)
Glucose solution (sterile)   2% (w/v)
Methotrexate 200 µg/mL
Amino acid mix (10×), without adenine     1×
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  1. doi:10.1101/pdb.rec090332 Cold Spring Harb Protoc 2016: pdb.rec090332- © 2016 Cold Spring Harbor Laboratory Press
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