Low-Fluorescence Yeast Nitrogen Base without Riboflavin and Folic Acid Medium (LFM) (10×)

Reagents	Final concentration (10×)
Yeast nitrogen base without riboflavin and folic acid	6.7 g/L
(NH₄)₂SO₄	5 g/L
KH₂PO₄	1 g/L
MgSO₄	0.5 g/L
NaCl	0.1 g/L
Ca₂Cl	0.1 g/L
H₃BO₄	0.5 mg/L
CuSO₄	0.04 mg/L
KI	0.1 mg/L
FeCl₃	0.2 mg/L
MnSO₄	0.4 mg/L
Na₂MoO₄	0.2 mg/L
ZnSO₄	0.4 mg/L
Biotin	2 µg/L
Calcium pantothenate	0.4 mg/L
Inositol	2 mg/L
Niacin	0.4 mg/L
Para-aminobenzoic acid (PABA)	0.2 mg/L
Pyridoxine hydrochloride	0.4 mg/L
Thiamine	0.4 mg/L
Galactose or glucose (optional; see below)	2% (w/v)
Amino acid mix (10×) (optional; see below)	1×
Always include sugars and amino acids (as appropriate) for cells that are not to be stressed while performing fluorescence microscopy or while doing the fluorometric or luminescent analyses. Store at 4°C. LFM without amino acids mix added can be stored at room temperature.