YPD Media (Liquid and Solid) for the OyCD PCA
| Reagents | Final concentration (1×) |
|---|---|
| Yeast extract | 1% (w/v) |
| Peptone | 2% (w/v) |
| Dextrose | 2% (w/v) |
| Agar (for solid medium only) | 2% (w/v) |
| G418 | 200 µg/mL |
| Nourseothricin | 100 µg/mL |
| 5-Fluorocytosine (5-FC) | 100 µg/mL |
| Prepare in Petri plates. For long term storage, keep at 2°C–8°C. | |
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