PBS-MK (10×)
| Reagent | Final concentration (10×) |
|---|---|
| Na2HPO4 (pH 7.4) | 100 mm |
| KH2PO4 | 18 mm |
| NaCl | as appropriate |
| MgCl2 | 10 mm |
| KCl | 25 mm |
| Sterilize 10× PBS-MK containing the appropriate concentration of NaCl using a disposable 0.22-µm vacuum filtration system in a tissue-culture hood and store at room temperature. When required, dilute to 1× in distilled water. | |
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