Recipe

DNase Dilution Buffer (10×)

Reagent Final concentration
Tris-HCl (pH 7.4) 250 mm
MgCl2 100 mm
Sterilize using a disposable 0.22-µm vacuum filtration system in a tissue-culture hood and store at room temperature.
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  1. doi:10.1101/pdb.rec091207 Cold Spring Harb Protoc 2016: pdb.rec091207- © 2016 Cold Spring Harbor Laboratory Press
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