Recipe

ChIP Buffer II

50 mm HEPES-KOH (pH 7.5)

500 mm NaCl

1% Triton

1 mm EDTA

0.1% (w/v) Sodium deoxycholate

Combine components and filter-sterilize. (This buffer can be stably stored for many months at room temperature.)

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  1. doi:10.1101/pdb.rec092650 Cold Spring Harb Protoc 2016: pdb.rec092650- © 2016 Cold Spring Harbor Laboratory Press

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