Enrichment of Mammary Basal and Luminal Cells for Cell-of-Origin Metastasis Studies
- 1Institut de Recherches Cliniques de Montréal, Montréal, Quebec H2W 1R7, Canada;
- 2Department of Medicine, Experimental Medicine Division, McGill University, Montréal, Quebec H3A 1A3, Canada;
- 3Pathologie et Biologie Cellulaire, Université de Montréal, Montréal, Quebec H3T 1J4, Canada;
- 4Université Pierre et Marie Curie, Université Paris 06; CNRS, Unité Mixte de Recherche 7211; INSERM, Unité Mixte de Recherche S959, I3, F-75013 Paris, France;
- 5University Paris Descartes, Sorbonne Paris Cité, Hôpital Necker-Enfants Malades, Faculté de Médecine Paris Descartes; INSERM, Unité 1013, F-75015 Paris, France;
- 6Laboratory of Molecular Immunology, Rockefeller University, New York, New York 10065
Abstract
The mammary gland is an important model system in metastasis research. Mammary epithelial stem cells are of particular interest because of their capacity for regeneration and their role in cancer initiation. This protocol describes how to enrich for mammary basal and luminal epithelial cells using fluorescence-activated cell sorting (FACS).
Footnotes
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↵7 Correspondence: podsypak{at}gmail.com
- © 2016 Cold Spring Harbor Laboratory Press
