Laemmli Loading Buffer (5×, pH 6.8)

• 2.5 mL 2 m Tris-HCL (pH 6.8)

• 2 g sodium dodecyl sulfate

• 100 mg bromophenol blue

• 10 mL glycerol

• 1.542 g DTT

Add up to 20 mL of dH₂O. Generate 100-μL aliquots of the buffer, and store them at −20°C for up to 3 mo.

• © 2016 Cold Spring Harbor Laboratory Press