Recipe

Protoplast Transformation Solution E

Tris–HCl 10 mm, pH 7.6
PEG 4000     20%
CaCl2     10 mm
Combine components in distilled water. Make fresh for each transformation.
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  1. doi:10.1101/pdb.rec092247 Cold Spring Harb Protoc 2016: pdb.rec092247- © 2016 Cold Spring Harbor Laboratory Press
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