Corrigendum: Growth and the Environment of Schizosaccharomyces pombe
Cold Spring Harb Protoc 2016; 10.1101/pdb.top079764
In earlier versions of this article, the recipe entitled “EMMG” did not specify that the monosodium salt of l-glutamic acid should be used. The authors apologize for this omission. A corrected version of the recipe is below. In addition, the HTML version of the recipe and the PDF version of the article have been amended accordingly.
EMMG
| Reagent | Quantity (for 1 L) | Final concentration |
|---|---|---|
| l-Glutamic acid, monosodium salt | 3.38 g | 20 mm |
| Potassium hydrogen phthalate | 3 g | 14.7 mm |
| Na2HPO4 | 2.2 g | 15.5 mm |
| Glucose | 20 g | 111 mm |
| Salt stock (50×) | 20 mL | 1× |
| Vitamins (1000×) | 1 mL | 1× |
| Minerals (10,000×) | 0.1 mL | 1× |
| H2O | to 1 L | |
| Prepare 1 L of EMMG by combining the reagents above. To prepare solid medium, include agar (20 g/L). Sterilize by autoclaving at 10 psi for 10 min (for liquid medium) or 15 min (for solid medium). (For liquid medium, low pressure and a short cycle is essential to avoid caramelization of glucose and breakdown of vitamins and minerals. For live cell imaging, filter-sterilized medium has lower background fluorescence than autoclaved medium. Use a 0.22-µm pore size for filtration.) Store at 4°C. If desired, add 12 µm thiamine (364 µL of a filter-sterilized stock solution of 10 mg/mL on H2O) after autoclaving to fully repress expression from nmt1-derived promoters (Maundrell 1990). | ||
- © 2016 Cold Spring Harbor Laboratory Press
