Erratum: Preparation of Nuclear Extracts from HeLa Cells
Cold Spring Harb Protoc 2013; 10.1101/pdb.prot075176
When this protocol was first published, the recipes entitled “Buffer B (Low Salt)” and “Buffer C (High Salt)” contained errors. The stock concentration of glycerol used in Buffer B (Low Salt) should have been expressed as “50%,” not as “50 mL,” and the final concentration of KCl in Buffer C (High Salt) should have been listed as “1.2 m,” not as “1.2 mm.” Corrected versions of these recipes are below, and the HTML versions of the recipes and the PDF version of the protocol have been amended accordingly.
Buffer B (Low Salt)
| Reagent | Quantity (for 100 mL) | Final concentration |
|---|---|---|
| Tris-HCl (1 m, pH 7.9) | 2 mL | 20 mm |
| Glycerol (50%, ultrapure) | 50 mL | 25% (v/v) |
| MgCl2 (1 m) | 0.15 mL | 1.5 mm |
| EDTA (0.5 m, pH 8.0) | 0.04 mL | 0.2 mm |
| KCl (3 m) | 0.66 mL | 20 mm |
| H2O | to 100 mL | |
| Store at 4°C. | ||
Buffer C (High Salt)
| Reagent | Quantity (for 100 mL) | Final concentration |
|---|---|---|
| Tris–HCl (1 m, pH 7.9) | 2 mL | 20 mm |
| Glycerol (ultrapure, 50%, v/v) | 50 mL | 25% (v/v) |
| MgCl2 (1 m) | 0.15 mL | 1.5 mm |
| EDTA (0.5 m, pH 8.0) | 0.04 mL | 0.2 mm |
| KCl (3 m) | 40 mL | 1.2 m |
| H2O | to 100 mL | |
| Store at 4°C. | ||
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