Protocol

Triggering Death of Adherent Cells with Ultraviolet Radiation

  1. Nigel J. Waterhouse1,2,3,4
  1. 1Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia;
  2. 2Flow Cytometry and Imaging, QIMR Berghofer Medical Research Institute, Herston, Brisbane, Queensland 4006, Australia;
  3. 3School of Medicine, University of Queensland, St. Lucia, Brisbane, Queensland 4072, Australia

    Abstract

    Ultraviolet (UV) radiation is a convenient stimulus for triggering cell death that is available in most laboratories. We use a Stratalinker UV cross-linker because it is a safe, cheap, reliable, consistent, and easily controlled source of UV irradiation. This protocol describes using a Stratalinker to trigger UV-induced death of HeLa cells.

    Footnotes

    • ↵4 Correspondence: nigel.waterhouse{at}qimrberghofer.edu.au

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    1. doi:10.1101/pdb.prot087148 Cold Spring Harb Protoc 2016: pdb.prot087148- © 2016 Cold Spring Harbor Laboratory Press
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