Sucrose Cutting Solution

In 1-L volumetric flask, combine the above reagents. Shake to prevent monobasic crystals from clumping. Fill the flask to ~950 mL with H₂O. Add MgCl₂ from a concentrated stock solution to a final concentration of 7 mm. (For example, add 3.5 mL of a 2 m MgCl₂ stock.) Add 1.32 mL of Calcium Chloride Stock Solution <R> for a final concentration of 0.5 mm CaCl₂. Top off the flask with H₂O to 1 L, and bubble the solution with carbogen for 10–20 min (which should resolve any cloudiness from the addition of calcium). Transfer the solution to a 1-L bottle and cap. Store the solution for up to 1 wk at 4°C.