EpiSC Medium
| Reagent | Amount | Final concentration |
|---|---|---|
| KnockOut D-MEM (Invitrogen 10829-018) | 385 mL | |
| KnockOut Serum Replacement (Invitrogen 10828-028) | 100 mL | 20% |
| Nonessential amino acids (10 mm stock; Invitogen 11140-050) | 5 mL | 0.1 mm |
| β-Mercaptoethanol (10 mm stock)a | 5 mL | 0.1 mm |
| GlutaMAX (200 mm stock; Invitrogen 35050-061) | 5 mL | 2 mm |
| Store the medium (final volume 500 mL) at 4°C and use within 1 mo. | ||
| aβ-Mercaptoethanol 10 mm stock is prepared from 14.3 m stock (Sigma-Aldrich M7522) by adding 7 µL to 10 mL of PBS. | ||
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