Protocol

Immunoprecipitation

  1. Thomas O. Kohl

Abstract

This immunoprecipitation protocol details individual steps for the enrichment and purification process of specific proteins from a complex cell lysate using an antibody bound to a solid matrix. Purified antigen(s) can be eluted by various methods, and the resultant protein target can be analyzed and/or identified by numerous assays, including the enzyme-linked immunosorbent assay (ELISA), western blotting, or mass spectrometry.

Footnotes

  • From the Antibodies collection, edited by Edward A. Greenfield.

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  1. doi:10.1101/pdb.prot098640 Cold Spring Harb Protoc 2017: pdb.prot098640- © 2017 Cold Spring Harbor Laboratory Press
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