RIPA Lysis Buffer
| Reagent | Final concentration |
|---|---|
| NaCl | 150 mm |
| Nonidet P-40 | 1% |
| DOC | 0.5% |
| SDS | 0.1% |
| Tris (pH 7.4) | 50 mm |
| Prepared RIPA buffer should be aliquoted and stored at −20°C. Add protease and/or phosphatase inhibitors to a thawed aliquot before immediate use. Discard and do not freeze again. One milliliter of buffer is sufficient to lyse approximately 5 million cells. | |
