Recipe

Staining Buffer for Xenopus Embryos

Reagent Final concentration
Tris-HCl (pH 9.5)  0.1 m
NaCl  0.1 m
MgCl2  50 mm
Tween 20  0.1%
Levamisole 0.065%
Prepare fresh in sterile milliQ H2O from filtered stock solutions.
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  1. doi:10.1101/pdb.rec102798 Cold Spring Harb Protoc 2017: pdb.rec102798- © 2017 Cold Spring Harbor Laboratory Press
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