| Aldrich K-gluconate (Sigma-Aldrich G4500) |
135 mm |
1.581 g |
| Aldrich EGTA (Sigma-Aldrich E0396) |
0.2 mm |
0.004 g |
| Aldrich HEPES (Sigma-Aldrich H4034) |
10 mm |
0.119 g |
| MgCl2 (1 m; Fluka 63020)
|
4 mm |
0.200 mL |
| Aldrich Na2-ATP (Sigma-Aldrich A3377)
|
4 mm |
0.121 g |
| Aldrich Na-GTP (Sigma-Aldrich G8877) |
0.4 mm |
0.010 g |
| Aldrich Na2-phosphocreatine (Sigma-Aldrich P7936)
|
10 mm |
0.128 g |
| Aldrich Ascorbate (l-ascorbic acid; Sigma-Aldrich A5960)
|
3 mm |
0.026 g |
| Adjust pH to 7.2 with KOH, and check osmolality (it should be 290–300 mOsm/kg). After mixing, filter-sterilize (0.2-µm pore
size) and divide into 100–500-µL aliquots. Store at −20°C.
|
