Recipe

Trypsin Versene Phosphate (TVP) Buffer

Reagent Amount
EDTA (Sigma-Aldrich E6758) 0.186 g
Chicken serum (Sigma-Aldrich C5405)   5 mL
Trypsin (2.5%) (Invitrogen 15090-046)   5 mL
PBS 500 mL
Filter, aliquot, and freeze at −20°C. Once thawed, do not refreeze; store at 4°C for up to 1 mo.
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  1. doi:10.1101/pdb.rec096099 Cold Spring Harb Protoc 2017: pdb.rec096099- © 2017 Cold Spring Harbor Laboratory Press
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