Figure 1.
SILAC-based phosphoproteomics workflow using light and heavy amino acids (AA); see Protocol: Construction, Growth, and Harvesting of Fission Yeast Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Strains (Koch et al. 2016) and Protocol: Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)-Based Quantitative Proteomics and Phosphoproteomics in Fission Yeast (Carpy et al. 2016). SCX, Strong cation exchange.
