RNase H (10 mg/mL) (DNase-Free)
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1. In a clean 15-mL Falcon tube, add
Bovine pancreatic RNase H 100 mg Sodium acetate (10 mm, pH 4.8) 9 mL -
2. Dissolve RNase crystals completely by vortexing.
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3. Place the tube in a boiling water bath for 15 min. Remove the tube and allow it to cool to room temperature (∼5 min).
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4. Add 1 mL of 1 m Tris-Cl and vortex for 30 sec.
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5. Five-milliliter aliquots of this solution may be stored at −20°C.
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RNase H is stable for 6 mo at −20°C.
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