P1 Buffer

• 1. In a clean 2-L beaker, add:

  H2O, autoclaved, distilled	920 mL
  Tris-Cl (1 m, pH 8.0)	50 mL
  EDTA (0.5 m, pH 8.0)	20 mL
  RNase H (10 mg/mL) (DNase-free)	10 mL

• 2. Add a sterile stir bar and stir for 5 min.

• 3. Filter and store at 4°C. Label the bottle with the date. Prepare fresh for each day of use.

• © 2017 Cold Spring Harbor Laboratory Press