P2 Buffer
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1. In a clean 1-L beaker, add:
H2O, autoclaved, distilled 880 mL NaOH (10 m) 20 mL SDS (10%) 100 mL -
2. Add a sterile stir bar and stir for 5 min. To avoid SDS precipitation, add the NaOH solution to the H2O and stir for few minutes before adding the SDS solution. Label the bottle with the date.
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P2 buffer should be made every 2 d and stored at room temperature to avoid SDS precipitation.
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3. If precipitation does occur, warm the bottle to 37°C and stir for a few minutes.
