Protocol

Ethyl Methanesulfonate Mutagenesis in Schizosaccharomyces pombe

  1. Geneviève Thon2
  1. 1Department of Biosciences and Nutrition, Karolinska Institute, Stockholm SE-141 83, Sweden;
  2. 2Department of Biology, University of Copenhagen, Copenhagen DK-2200, Denmark
  1. ↵3Correspondence: karl.ekwall{at}ki.se

Abstract

Here we provide an ethyl methanesulfonate (EMS) mutagenesis protocol for Schizosaccharomyces pombe cells.

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This Article

  1. doi:10.1101/pdb.prot091736 Cold Spring Harb Protoc 2017: pdb.prot091736- © 2017 Cold Spring Harbor Laboratory Press
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Subject Categories

  1. Yeast Genetics
  2. Yeast
  3. Mutagenesis

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