Live Imaging of Chromosome Segregation during Meiosis in the Fission Yeast Schizosaccharomyces pombe
- 1Laboratory of Cell Responses, National Institute for Basic Biology, Okazaki, Aichi 444-8585, Japan;
- 2Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Okazaki, Aichi 444-8585, Japan;
- 3Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Tokyo 113-0032, Japan
- ↵5Correspondence: ymst{at}nibb.ac.jp; yamamoto{at}nibb.ac.jp
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↵4 These authors contributed equally to this work.
Abstract
This protocol describes the live observation of chromosome segregation during fission yeast meiosis. To visualize one chromosome of interest, the lac operator (lacO array) is integrated at its centromere-proximal locus, and the lac repressor (lacI)-GFP fusion protein is expressed in a haploid strain. This haploid strain, in which mCherry-tagged tubulin is also expressed exogenously to monitor meiotic progression, is crossed with a nonlabeled haploid strain to induce meiosis. GFP and mCherry signals in resulting zygotes are observed by a fluorescent microscopy during the progression of meiosis.
Footnotes
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From the Fission Yeast collection, edited by Iain M. Hagan, Antony M. Carr, Agnes Grallert, and Paul Nurse.
