RGL Basal Medium
| Reagent | Amount |
|---|---|
| DMEM (e.g., Sigma-Aldrich catalog no. D6546) | 24.25 mL |
| Nutrient Mixture F-12 Ham (e.g., Sigma-Aldrich catalog no. D4888) | 24.25 mL |
| Glutamine solution (0.2 m) (e.g., Sigma-Aldrich G 1626) | 0.5 mL |
| B-27 supplement (50×) (Invitrogen catalog no. 17504044) | 1 mL |
| Combine in a 50-mL conical tube. Mix well. | |
