Complete ES Cell Medium for 129S6/SvEvTac×C57BL/6N F1 Hybrid ES Cells
Supplement DMEM (Invitrogen catalog no. 11971) with the following:
| Reagent | Final concentration |
|---|---|
| FBS (ES cell tested; Hyclone) | 15% |
| l-glutamine | 2 mm |
| Nonessential amino acids | 0.1 mm |
| Sodium pyruvate | 1 mm |
| β-mercaptoethanol | 0.1 mm |
| Penicillin and streptomycin | 50 µg/mL each |
| LIF (Millipore) | 2000 U/mL |
