PXL Wash Buffer (1×)
| Reagent | Quantity (for 1 L) | Final concentration |
|---|---|---|
| PBS (10×) (tissue culture grade; no Mg2+, no Ca2+) | 100 mL | 1× |
| SDS (20%) | 5 mL | 0.1% (v/v) |
| Sodium deoxycholate | 5 g | 0.5% (w/v) |
| Nonidet P-40 (NP-40) | 5 mL | 0.5% (v/v) |
| RNase-free SDS is purchased as a 20% solution. Make up to 1 L with water. Store at 4°C. | ||
