PK Buffer (1×)/Urea (7 m)
| Reagent | Quantity (for 10 mL) | Final concentration |
|---|---|---|
| Tris-HCl (1 m, pH 7.5) | 1 mL | 100 mm |
| NaCl (5.0 m) | 0.1 mL | 50 mm |
| EDTA (0.5 m, pH 8.0) | 0.2 mL | 10 mm |
| Urea | 4.2 g | 7 m |
| Make up to 10 mL with water. This buffer must be made fresh each time. | ||
