Recipe

DMSO-Only Control Solution

Reagent Amount (1 L) Final concentration
DMSO 0.2 mL  0.2%
BrdU (10 mm in frog water)   0.1 L 1 mm
Frog water
Prepare fresh on the day of use in glass bottles covered in foil. Add DMSO to frog water. Add 10 mm BrdU stock solution to a final concentration of 1 mm. Stir to mix.
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  1. doi:10.1101/pdb.rec104810 Cold Spring Harb Protoc 2018: pdb.rec104810- © 2018 Cold Spring Harbor Laboratory Press
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