Recipe

Spheroplasting Buffer

Reagent Volume
Citrate phosphate (0.2 m, pH 5.6)  5 mL
EDTA (0.5 m, pH 8.0)  5 mL
Sorbitol (2 m) 25 mL
Sterile H2O 15 mL
Store for up to 1 mo at room temperature.
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  1. doi:10.1101/pdb.rec094110 Cold Spring Harb Protoc 2018: pdb.rec094110- © 2018 Cold Spring Harbor Laboratory Press
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