Recipe

DNA Combing Plug Solution

Spheroplasting buffer  1 mL
Zymolyase 20T 10 mg
Lysing enzyme (Sigma-Aldrich L1412) 12 mg
β-mercaptoethanol  10 µL
Make fresh for each application.
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  1. doi:10.1101/pdb.rec094128 Cold Spring Harb Protoc 2018: pdb.rec094128- © 2018 Cold Spring Harbor Laboratory Press
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