Recipe

Elution Buffer

Reagent Stock solution Quantity (for 50 mL) Final concentration
Tris (pH 8.0)  1 m   500 µL 10 mm
EDTA 0.5 m   100 µL  1 mm
SDS  10%   5 mL   1%
dH2O 44.4 mL
This buffer can be prepared in advance and stored.
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  1. doi:10.1101/pdb.rec083006 Cold Spring Harb Protoc 2018: pdb.rec083006- © 2018 Cold Spring Harbor Laboratory Press

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