Elution Buffer
| Reagent | Stock solution | Quantity (for 50 mL) | Final concentration |
|---|---|---|---|
| Tris (pH 8.0) | 1 m | 500 µL | 10 mm |
| EDTA | 0.5 m | 100 µL | 1 mm |
| SDS | 10% | 5 mL | 1% |
| dH2O | 44.4 mL | ||
| This buffer can be prepared in advance and stored. | |||
| Reagent | Stock solution | Quantity (for 50 mL) | Final concentration |
|---|---|---|---|
| Tris (pH 8.0) | 1 m | 500 µL | 10 mm |
| EDTA | 0.5 m | 100 µL | 1 mm |
| SDS | 10% | 5 mL | 1% |
| dH2O | 44.4 mL | ||
| This buffer can be prepared in advance and stored. | |||