Recipe

Buffer L1

Reagent Stock solution Quantity (for 60 mL) Final concentration
HEPES (pH 7.5)  1 m   3 mL  50 mm
NaCl  5 m  1.68 mL 140 mm
EDTA (pH 8.0)  0.5 m  120 µL   1 mm
Glycerol 100%    6 mL    10%
NP-40 100%    3 mL  0.5%
Triton X-100 100%  0.15 mL  0.25%
dH2O 46.05 mL
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  1. doi:10.1101/pdb.rec083030 Cold Spring Harb Protoc 2018: pdb.rec083030- © 2018 Cold Spring Harbor Laboratory Press
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